![]() ![]() We used a human peripheral blood lymphocyte separator (purchased from Beijing Solarbio Technology Co., Ltd.) to separate PBMCs, extracted the total RNA by Trizol method, and determined the purity and concentration via ultraviolet spectrophotometer. In order that the early diagnosis of DVT in elderly patients after total hip arthroplasty can be further improved, this study explored and analyzed the miR-374a-5p in PBMCs for predicting DVT in elderly patients after total hip arthroplasty. Scholars consider that the circulating miRNAs may be a new biomarker for DVT in elderly patients with lower limb surgery. miRNAs can exist stably in the systemic circulatory system. It binds to mRNA by base pairing, and regulates protein expression by inhibiting mRNA translation. ![]() miRNA is a type of highly conserved non-coding single-stranded RNA (around 22 bases). Studies have proposed that early diagnosis and treatment of lower extremity thrombosis can effectively reduce the incidence of pulmonary embolism, and is of significant value in improving the treatment prognosis. For severe cases, the thrombus may separate to cause pulmonary embolism, which endangers the patient’s life. However, deep vein thrombosis (DVT) of lower extremities often occurs due to the large surgical trauma caused by hip replacement and the necessity for bed-rest after surgery. Hip arthroplasty is a common surgical method used clinically to treat hip diseases. Hip joint disease is one of the most frequent orthopedic diseases in the elderly, and damage to it can cause serious affects to daily life. Its expression level has a critically positive correlation with D-dimer degree, which is likely to be abiologic indicator for the early diagnosis and treatment of DVT. Conclusion: miR-374a-5p is of important value in the prediction of DVT in elderly patients with total hip arthroplasty. The correlation analysis indicated that miR-374a-5p was extremely positively correlated with D-dimer ( P<0.05). The areas under the predictive curve of D-dimer for DVT was 0.470, 0.834, 0.863 and 0.790 respectively on 1 d pre-surgery, and 1 d, 3 d and 5 d post-surgery. We analyzed the predictive value of miR-374a-5p for DVT by ROC curve, and the areas under the predictive curve of miR-374a-5p on 1 d pre-surgery, and 1 d, 3 d and 5 d post-surgery for DVT were 0.435, 0.820, 0.974 and 0.782 respectively. Results: The relative expression of miR-374a-5p in PBMCs and plasma D-dimer of the two groups 1 d, 3 d, and 5 d after surgery were higher than those on the 1 d before surgery ( P<0.05), and the indexes of thrombus-group-patients were higher than those in the non-thrombosis group at various time points after surgery ( P<0.05). We detected the expression levels of miR-374a-5p in PBMCs and plasma D-dimer of the two groups of patients 1 d before surgery, and 1 d, 3 d, and 5 d after surgery, and analyzed the predictive value, and the correlation between miR-374a-5p and plasma D-dimer for DVT by ROC curve. According to the color Doppler ultrasound of lower limbs 5 d postoperatively, subjectswere classified into thrombosis group (n=31) and non-thrombosis group (n=81). Methods: There was a total of 112 elderly patients that underwent elective total hip arthroplasty in our hospital and were enrolled as the research subjects. Objective: This study analyzed the predictive value of peripheral blood mononuclear cells’ (PBMCs) miR-374a-5p in deep vein thrombosis (DVT) after total hip arthroplasty in the elderly.
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